Coding

Part:BBa_K3757001:Design

Designed by: Erik Zimmer   Group: iGEM21_Tuebingen   (2021-09-25)


Oak1 precursor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA sequence has been changed to allow recognition and cyclization of the expressed peptide by CtAEP1. DNA sequence has been modified for optimized usage in Nicotiana benthamiana. Includes lacZ operon for blue-white selection.


Source

From Oldenlandia affinis, sequence from GenBank: AF393825.1

References