Coding
Part:BBa_K3757001:Design
Designed by: Erik Zimmer Group: iGEM21_Tuebingen (2021-09-25)
Oak1 precursor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 625
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
DNA sequence has been changed to allow recognition and cyclization of the expressed peptide by CtAEP1. DNA sequence has been modified for optimized usage in Nicotiana benthamiana. Includes lacZ operon for blue-white selection.
Source
From Oldenlandia affinis, sequence from GenBank: AF393825.1